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BEIRUT – The Syrian opposition on Wednesday accused the government of launching a chemical weapons attack in the Damascus suburbs that killed scores of civilians as they slept and left makeshift hospitals packed with hundreds of victims convulsing and gasping for breath.

Photographs and videos posted online showed the bodies of men, women and children, many appearing to be dead, without visible wounds. Varying opposition claims put the death toll in the hundreds, with some saying it was more than a thousand.

While the exact cause of the deaths remained unclear, there was widespread agreement, as one European expert noted, that “something terrible has happened.”

If confirmed, the use of toxic agents would be the most serious in decades, presenting a renewed challenge to President Obama, who said last year that the use of chemical weapons in Syria would constitute a “red line” requiring a U.S. response.

The Syrian government strongly denied responsibility. But a White House statement put the blame squarely on Syrian President Bashar Assad, referring to “reports that hundreds of Syrian civilians have been killed in an attack by Syrian government forces, including by the use of chemical weapons.”

The deaths touched off a vigorous debate among independent experts about whether available information pointed to the use of a well-known chemical weapon such as the nerve agent sarin or to some other toxic agent. U.S. intelligence agencies offered no early verdict.

The attack came as U.N. investigators were already on the ground in Syria to investigate previous incidents in which the United States and its allies have said small quantities of chemical weapons were used. But the U.N. Security Council was unable to agree Wednesday on whether those experts should immediately investigate the new incident.

The Russian government, Assad’s strongest supporter, suggested that the opposition itself had staged the attack in a “pre-planned provocation.’’

Sama Masoud, an opposition activist who lives in one of the targeted neighborhoods, described scenes of chaos on the streets, as panicked residents did not know whether to stay in their homes or flee.

“The fiancé of my sister has died,” she said. “My friend, her husband and her husband’s uncle – all dead while asleep.”

More than 130 videos were posted online showing the victims. In some, children lay on tiled floors, vomiting, convulsing and struggling to breathe as they were treated with hand-held respirators or as medics desperately administered chest compressions.

In others, men sprawling on the floor of a makeshift hospital are hosed down with water, in what appeared to be a desperate attempt to wash off the remnants of the poisonous gases.

Dr. Majed Abu Ali, a medic in nearby Douma, said his team had treated 600 patients with symptoms including reddened eyes with constricted pupils, vomiting, skin rashes, loose bowels and extreme problems breathing. He said 65 had died and that “overwhelmed” medical staff had little to protect themselves from toxins.

Some experts questioned the video evidence. “It strikes me as odd that the people who are treating the infected victims are not undressing them,” said Raymond Zilinskas, a biologist and former U.N. weapons inspector in Iraq. “When you’re dealing with a chemical event, there is always a ‘red zone’ and a ‘green zone’ – always,” to protect medical providers.

But others said the symptoms exhibited by victims were far more convincing than in any of the previous alleged chemical incidents.

Amy Smithson, a senior fellow at the James C. Martin Center for Nonproliferation Studies, said the photographic evidence “clearly indicates exposure to a toxic chemical,” citing a combination of tell-tale symptoms such as respiratory problems and twitching, and the near absence of wounds that would be associated with conventional explosives. But she acknowledged that it was impossible to tell if the apparent poisoning was caused by sarin or one of the other known toxins in Syria’s arsenal.

“Regardless of whether this was a classic warfare agent like sarin, the Chemical Weapons Convention outlaws use of any toxic chemical for military purposes,” Smithson said.

Jean-Paul Zanders, an expert on chemical and biological weapons at the European Union Institute for Security Studies in Paris, said in a blog posting that the photographs appeared to confirm “exposure to toxin,” but not necessarily nerve gas. He added, “It is clear that something terrible has happened. These scenes could not have been stage-managed.”

Even the more conservative opposition casualty estimates of Wednesday’s attack would make it the most extensive use of chemical weapons since 1988, when thousands of Iraqi civilians were killed in the Kurdish village of Halabja in an attack launched by President Saddam Hussein.

At a news conference in Istanbul, George Sabra, deputy head of the Syrian Opposition Coalition, said the death toll has reached 1,300, with chemical strikes also hitting the Western Ghouta neighborhood of Moadamyet al-Sham. Denouncing the lack of international action on Syria, Sabra reiterated calls for a no-fly zone, saying that not only is the Assad regime killing the Syrian people, “the weakness of the U.N. is killing us. The U.S. hesitation is killing us.”